251
E Diagnostics quick reference
Dispense task parameters
Bravo Platform User Guide
Dynamic tip retraction
(0–20 mm/µL)
The rate at which to raise the pipette head during
the Dispense task.
Use dynamic tip retraction to prevent spills as the
pipette tips displace the liquid.
To move the tips:
• At the same rate as the volume change.
Calculate dynamic tip retraction (DTR) as
follows:
DTR = (well depth)/(well vol) = 1/A, where A is
the cross-sectional area of a well with straight
walls
• Faster than the volume change.
DTR > 1/A
• Slower than the volume change.
DTR < 1/A
The starting and ending positions can be
calculated as follows:
(V
dispensed
* DTR) + Distance
well bottom
Pipette technique The pipette location offset you want to use for the
Dispense task.
The list of pipette techniques are defined in the
Pipette Technique Editor.
Perform tip touch The option to touch the pipette tip on one or more
sides of the well.
Which sides to use for tip touch The side or sides of the well to use during tip
touch: North, South, East, West, North/South,
West/East, West/East/South/North.
Tip touch retract distance
(–20 to 50 mm)
The vertical distance for the pipette tips to rise
before touching the sides of the wells.
Tip touch horizontal offset
(–5 to 5 mm)
The horizontal distance the tips move. The value
is based on the well diameter specified by the
labware definition.
For example, if you set a value of:
• 0, the tips move a horizontal distance equal to
the well radius
• > 0, the tips attempt to move past the well
radius, which results in a more forceful tip
touch
• < 0, the tips move a distance less than the
radius of the well, resulting in a lighter tip
touch
Parameter Description
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