150 BD FACSCanto II Instructions for Use
3 In Basic Controls, click Acquire Data.
4 After 300 sec (5 min), click and remove the tube.
5 Install a tube with 3 mL of DI water on the SIT. Firmly push up on the
tube until it comes to a complete stop and is fully seated.
6 Repeat steps 3 and 4.
7 Choose Cytometer > Fluidics Shutdown, and then follow all prompts.
8 Turn off cytometer power.
BD Biosciences recommends turning off the cytometer when not in use to
preserve laser life.
NOTICE After every 4 hours of operation, and during cytometer
shutdown, you will hear a hiss as condensed water discharges from the
fluidics cart pumps into the condensation trap.
9 Quit BD FACSDiva software.
The following dialog appears. Verify Exit application only is selected and
click OK.
To prevent fluid overflow, do not leave a tube on the SIT during fluidics
shutdown.
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