BD FACSCanto II - Page 19

Chapter 1: Introduction 19
Blank optical holders do not contain optical filters. They are used in the trigon
and octagon to prevent unwanted light from interfering with fluorescence signal.
Table 1-1 Trigon and octagon optical filters (4-2 configuration)
Detector Array
(Laser)
PMT
Position
LP Mirror
BP Filter or
LP Mirror
Intended Dye
Octagon
(488-nm blue laser)
A 735 780/60 PE-Cy™7
B 655 670 PerCP-Cy™5.5
or PerCP
C 610 blank
optical holder
—
D 556 585/42 PE
E 502 530/30 FITC
Fblank
optical holder
488/10
and pinhole
SSC
Gblank
optical holder
blank
optical holder
—
H— blank
optical holder
—
Trigon
(633-nm red laser)
A 735 780/60 APC-Cy7
B 685 blank
optical holder
—
C — 660/20 APC