Chapter 5: Cytometer QC and Setup 73
Figure 5-6 Adjusting Threshold
3 (Optional) Enable and set another threshold parameter.
• Select the associated checkbox.
• Edit the threshold value.
• Select Or (any of the parameter thresholds can be met) or And (all of
the parameter thresholds must be met).
Adjusting Spectral Overlap
Fluorochromes emit light over a range of wavelengths. During cytometer setup,
fluorescence spillover is automatically determined and corrected. If necessary,
you can use the spectral overlap controls to make manual adjustments:
1 Click in the Spillover % value field that needs correction (Figure 5-7 on
page 74).
2 Use one of the editing methods specified in Adjusting Detectors on page 71.
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