Hologic thinprep 2000 - FIXATION

8
FIXATION, STAINING, AND COVERSLIPPING
8.2 ThinPrep 2000 Processor Operator’s Manual
FIXATION
The ThinPrep 2000 processor deposits completed slides into a fixative vial that contains 95% reagent
alcohol or 95% ethyl alcohol. Use the following procedure to fix ThinPrep microscope slide
preparations.
1. Remove each slide after it is deposited into the fixative vial in the ThinPrep 2000 processor.
2. Place the slide in a multi-slide holder and place the slide holder into a fixative bath containing
95% reagent alcohol or 95% ethyl alcohol. In order to minimize exposure of ThinPrep microscope
slides to air:
• When transferring ThinPrep microscope slides from the fixative vial to the multi-slide fixa-
tive container, care should be taken to perform this operation quickly.
• If ThinPrep microscope slides are being transferred to a staining rack, care should be taken
that ThinPrep slides are continuously immersed in fixative.
3.
Gyn slides: ThinPrep microscope slides should be fixed for at least 10 minutes prior to staining.
Non-Gyn slides: ThinPrep microscope slides should be fixed for at least 10 minutes prior to
staining or application of fixative spray.
For Gyn slides intended for use with the ThinPrep
®
Imaging System: If the slides must be
shipped to another site prior to staining,
CellFyx™ Solution fixative must be applied.
Note: No other spray fixative has been validated for use with the ThinPrep Imaging System.
Contact Hologic Customer Service for ordering. See the instructions for use that come
with the fixative solution.
SECTION
B