NON-GYNECOLOGIC SAMPLE PREPARATION
1.12
ThinPrep 2000 Processor Operator’s Manual
ADD SPECIMEN TO PRESERVCYT SOLUTION VIAL
Determine the cell pellet size and refer to the table below:
Factors to Consider
The type of pipette that you use may affect the concentration of the sample that is added to the
PreservCyt Solution vial, and therefore may affect the volume of sample. Hologic recommends using
standard, 1 mL, graduated, plastic pipettes.
If a “Sample Is Dilute” message occurs repeatedly and specimen remains in the specimen tube,
increase the number of drops of concentrated sample added to the vial.
Your technique for pouring off the supernatant may also affect the concentration of the sample. If the
supernatant is not completely poured off, then additional drops of the sample may be required. The
total volume added to the vial must not exceed 1mL.
Size of Cell Pellet Procedure
Pellet is clearly visible
and the pellet volume is
less than 1 mL.
Place the centrifuge tube in a vortexor to resuspend the
cells in the residual liquid or mix the pellet by syringing it
manually with a pipette.
Transfer 2 drops of the pellet to a fresh PreservCyt Solu-
tion vial.
Pellet is not visible or is
scant.
Add the contents of a fresh PreservCyt Solution vial
(20 mL) into the tube.
Vortex briefly to mix the solution and pour the entire sam-
ple back into the PreservCyt Solution vial.
Pellet volume is greater
than 1 mL.
Add 1 mL of CytoLyt Solution into the tube. Vortex
briefly to resuspend the pellet. Transfer
1 drop
of the
specimen to a fresh PreservCyt Solution vial.
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