Hologic thinprep 2000 - SECTION D-9: Cytolyt Solution Wash

1
NON-GYNECOLOGIC SAMPLE PREPARATION
1.16 ThinPrep 2000 Processor Operator’s Manual
CYTOLYT SOLUTION WASH
Addition of CytoLyt Solution to cell pellets is required to wash the sample. A CytoLyt Solution Wash
performs the following functions while preserving cellular morphology:
• Lyse red blood cells
• Dissolve mucus
• Reduce protein precipitation
A
CytoLyt Solution Wash consists of the following process:
• Adding 30 mL of CytoLyt Solution to a cell pellet
•
Mucoid Specimens Only: mechanical agitation
• Concentration by centrifugation — 600g x 10 minutes
• Pouring off the supernatant and vortexing to resuspend the cell pellet
One
CytoLyt Solution Wash is usually adequate to clean most non-gyn samples. For particularly
bloody or mucoid specimens, additional
CytoLyt Solution Washes may be necessary.
When a sample is collected in CytoLyt Solution at a ratio less than 30 parts CytoLyt Solution to 1 part
sample, this is considered a
Collection Step and not a Wash Step . For example, if one collects 15 mL of a
sample and adds 30 mL of CytoLyt Solution to this sample, then the CytoLyt: sample ratio is only 2 to
1 and this is considered a sample collection step and still requires a
CytoLyt Solution Wash .
For more information on CytoLyt Solution, refer to Chapter 2, “Solutions”.
SECTION
D-9
30 ml
MUCOID
ONLY