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Hologic thinprep 2000 - Sample Preparation Troubleshooting

Hologic thinprep 2000
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NON-GYNECOLOGIC SAMPLE PREPARATION
ThinPrep 2000 Processor Operator’s Manual
1.27
1
SAMPLE PREPARATION TROUBLESHOOTING
Because there is biological variability among samples and variability in collection methods, standard
processing may not always yield a satisfactory and uniformly distributed preparation on the first
slide. This section contains instructions for further sample processing to obtain better quality subse-
quent slides in these cases.
After staining, you may observe the following irregularities:
Non-uniform distribution of the cells in the cell spot that was not accompanied by a “Sample
Is Dilute” message.
Uneven distribution in the form of a ring or “halo” of cellular material and/or white blood
cells
A sparse cell spot lacking in a cellular component and containing blood, protein, and debris.
This type of slide may be accompanied by a “Sample Is Dilute” message.
Note:
Satisfactory slide appearance is a matter of judgment and experience. Hologic recommends
that you check the quality of the slide after staining. If you determine that the slide is unsatis-
factory, use the procedures in this section to make additional slides.
Caution:
Be sure to use a new Non-Gynecologic filter for each slide.
SECTION
G

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