NON-GYNECOLOGIC SAMPLE PREPARATION
ThinPrep 2000 Processor Operator’s Manual
1.21
BODY FLUIDS
Body fluids may include serous effusions, urinary and cerebrospinal fluids.
1. Collection: Collect body fluids fresh.
Note:
Fluids collected in CytoLyt Solution also require a CytoLyt
Solution wash prior to instrument processing.
Note:
For extremely bloody fluids (i.e., pericardial), start with only
10 mL of fresh fluid.
Note:
Urine may be collected into PreservCyt Solution utilizing the
ThinPrep
®
UroCyte Urine Collection Kit. (Refer to Section F for
details.)
2. Concentrate by centrifugation — 600g for 10 minutes.
3. Pour off supernatant and vortex to resuspend cell pellet.
4. CytoLyt Solution wash
5. Evaluate cell pellet appearance.
Refer to page 1.11.
If cell pellet is not free of blood, add 30 mL of CytoLyt Solution to
cell pellet and repeat from step 2.
6. Add appropriate amount of specimen to PreservCyt Solution vial.
Refer to page 1.12.
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