NON-GYNECOLOGIC SAMPLE PREPARATION
1.24
ThinPrep 2000 Processor Operator’s Manual
THINPREP
®
UROCYTE
®
SPECIMENS
Your ThinPrep 2000 processor does not currently support UroCyte sample preparation. In order to
perform urine cytology using this system, additional software must be obtained. Please refer to
the Ordering Information in this manual or call Customer Service at 1-508-263-2900 for more
information.
(For use with urine cytology processing or slide-based molecular testing.)
1. Collection. Collect urine directly into the ThinPrep UroCyte Urine
Collection Kit,
OR
process urine fresh.
Note:
Fresh urine can be mixed with a 2:1 urine-to-PreservCyt
®
Solution
ratio and stored for up to 48 hours before processing.
Note:
If using the UroCyte Urine Collection Kit, do not exceed a 2:1
ratio of urine to PreservCyt Solution. If the urine volume exceeds
60 mL, pour off excess. A minimum volume of 33 mL of urine is
required to perform the Vysis
®
UroVysion assay.
2. Concentrate by centrifugation.
Transfer the sample evenly into two labeled to 50-mL centrifuge
tubes.
Centrifuge at 600g for 10 minutes.
3. Pour off supernatant and resuspend cell pellet.
Resuspension can be done on a vortexor or may be achieved by
syringing the pellet back and forth with a plastic pipette.
4. CytoLyt
®
Solution Wash
Add 30 mL of CytoLyt Solution to one 50-mL centrifuge tube and
vortex. Transfer the contents of this tube into the second 50-mL cen-
trifuge tube and vortex. The specimen is now combined into one 50-
mL tube. The empty tube can be discarded.
Centrifuge.
Pour off supernatant.
Resuspend cell pellet.
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