NON-GYNECOLOGIC SAMPLE PREPARATION
1.8
ThinPrep 2000 Processor Operator’s Manual
CONCENTRATE BY CENTRIFUGATION — 600g for 10 Minutes
The purpose of this procedure is to concentrate the cellular material in order to
separate the cellular component(s) from the supernatant. This step is performed
with fresh samples and after the addition of CytoLyt Solution. When specified in
the protocol, centrifuge samples at 600 times normal gravity (600g) for 10 minutes
to force the cells in solution into a pellet at the bottom of the centrifuge tube.
Set your centrifuge to the approximate number of revolutions per minute (rpm)
to spin the cells at 600g.
Follow these steps to determine the correct setting for your centrifuge:
CAUTION:
Check cell morphology on non-critical experimental samples before making any
changes to your centrifugation process.
Note:
Use of fixed-angle centrifuges is not recommended.
Measure the rotor length of your centrifuge.
Use a centimeter ruler to measure the radius of your centrifuge, the distance from the center of the
rotor to the bottom of the bucket extended horizontally as shown in Figure 1-1.
Figure 1-1 Measuring the Centrifuge
Refer to the chart in Figure 1-2.
Find the radius of your centrifuge in the first column of Figure 1-2. Draw a line from the radius value
through the 600 Gravities (g) column and into the rpm column. Read the rpm value from the straight
edge as shown in Figure 1-2. Run your centrifuge at this speed to achieve a force of 600g on your
samples.
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