NON-GYNECOLOGIC SAMPLE PREPARATION
ThinPrep 2000 Processor Operator’s Manual
1.5
COLLECTION
Note:
The ThinPrep
®
2000 processor is designed for use with PreservCyt
®
Solution. Do not run any
other collection media through it.
Samples to be processed on the ThinPrep processor will arrive in the lab either fresh or in CytoLyt
Solution. There are preferred collection methods for different sample types. This section will describe
the Hologic-recommended procedure as well as alternate collection methods.
WARNING:
For washes and lavages, do not expose the patient to CytoLyt Solution.
Fine Needle Aspirate Specimens:
The optimal collection technique for FNAs is to deposit and rinse the entire sample
into a centrifuge tube containing 30 mL of CytoLyt Solution. A secondary method
would be to collect the sample into a balanced electrolyte solution, such as Polysol
®
or Plasma-Lyte
®
injection solutions.
Note:
Direct smears may be necessary for radiologic-guided FNAs when a rapid analysis of speci-
men adequacy is required.
Mucoid Specimens:
Mucoid specimens, sputa and brushes, are best collected into CytoLyt Solution. If
they are collected fresh, CytoLyt Solution should be added as soon as possible. Early
addition of CytoLyt Solution preserves the sample and initiates the mucus dissolu-
tion process.
Large volume of fresh mucoid specimens (greater than 20 mL) should be concen-
trated before addition of CytoLyt Solution to the sample.
D-8. Mechanical agitation (mucoid samples only, optional)
D-9. CytoLyt Solution wash
(Some specimens do not require a CytoLyt wash. Refer to the
specific specimen preparation protocol.)
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