NON-GYNECOLOGIC SAMPLE PREPARATION
1.6
ThinPrep 2000 Processor Operator’s Manual
Fluid Specimens:
The preferred method for preparing fluid samples (urinary tract, effusions,
synovial, and cyst fluids) is to concentrate the fresh sample before any addi-
tion of CytoLyt Solution. If this is not possible and the samples must be
preserved for transport to the lab, collect the samples in CytoLyt Solution.
CytoLyt Solution added directly to fluids with high levels of protein may produce
some degree of protein precipitation.
Note:
Fluid collection in CytoLyt Solution is only considered a collection step and not a
wash step. See “CYTOLYT SOLUTION WASH” on page 1.16, in this section for more
detail.
The quantity of fluid samples can vary widely from less than 1 mL to 1000 mL and more.
Each lab must follow its own procedure for determining the amount of sample to use for pro-
cessing. If more than one centrifuge tube of sample is used, the cell pellets can be combined
after pouring off the supernatant.
Superficial Specimens:
Superficial brushings and scrapings are the only non-gynecologic samples
which are collected directly into PreservCyt Solution.
Other Collection Media:
In cases where CytoLyt Solution is contraindicated, balanced electrolyte solutions, such as
Plasma-Lyte and Polysol, may be used as collection media for samples to be processed on the
ThinPrep 2000 processor. These solutions are primarily used as media for washings or
lavages which contact the patient.
Non-Recommended Collection Media:
Hologic does not recommend the use of the following collection solutions with the ThinPrep
System. Use of these solutions will produce sub-optimal results:
• Sacomanno and other solutions containing carbowax
• Alcohol
• Mucollexx
®
• Normal saline
• Culture media, RPMI solution
•PBS
• Solutions containing formalin
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