NON-GYNECOLOGIC SAMPLE PREPARATION
ThinPrep 2000 Processor Operator’s Manual
1.19
MUCOID SPECIMENS
Mucoid specimens may include respiratory and gastrointestinal specimens.
1. Collection:
Collect sample directly into 30 mL of CytoLyt Solution. OR
Add 30 mL of CytoLyt Solution to the fresh specimen as soon as
possible.
Note:
Large specimens (greater than 20 mL) should be concentrated
before addition of CytoLyt Solution to the sample.
Optional: If DTT is being used with respiratory mucoid samples, add stock
before agitation. See the following page for preparation instructions.
2. Mechanical agitation
Note:
Vortex for a minimum of 5 minutes in “hands-free” vortexor.
3. Concentrate by centrifugation — 600g for 10 minutes.
4. Pour off supernatant and vortex to resuspend cell pellet.
5. Evaluate cell pellet appearance.
Refer to page 1.11.
Confirm the cell pellet is in liquid form. If the cell pellet is not in
liquid form, add 30 mL of CytoLyt Solution and repeat steps 2–4.
6. Add appropriate amount of specimen to PreservCyt Solution
vial.
Refer to page 1.12.
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