NON-GYNECOLOGIC SAMPLE PREPARATION
1.20
ThinPrep 2000 Processor Operator’s Manual
Procedure for the Use of DiThioThreitol (DTT) with Mucoid Non-Gyn
Samples
DTT has been shown to be a reagent that is effective in reducing the amount of mucus in
respiratory samples.
1,2
DTT Stock Solution
• Prepare a stock solution by adding 2.5 g DTT
3
to 30 mL of CytoLyt Solution.
• This solution is suitable for use for 1 week when stored at room temperature (15°C–
30°C).
Sample Preparation
• This procedure is designed for mucoid non-gyn sample processing. Follow the steps
for processing mucoid specimens on the previous page.
• After sample collection (Step 1), but prior to vortexing (Step 2), add 1 mL of the stock
DTT solution to the sample.
• Proceed with the remaining sample processing steps as listed.
1 Tockman, MS et al., ‘Safe Separation of Sputum Cells from Mucoid Glycoprotein’ Acta
Cytologica 39, 1128 (1995).
2 Tang, C-S, Tang CMC and Kung, TM, ‘Dithiothreitol Homogenization of Prefixed Spu-
tum for Lung Cancer Detection’, Diagn. Cytopathol. 10, 76 (1994).
3 Available from Amresco, contact a sales representative at 800-448-4442 or
www.amresco-inc.com.
7. Allow to stand in PreservCyt Solution for 15 minutes.
8. Run on ThinPrep 2000 processor using
Sequence 3
, Fix, Stain,
and Evaluate.
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