GYNECOLOGIC SAMPLE PREPARATION
4.8
ThinPrep 2000 Processor Operator’s Manual
Reprocessing Protocol
1 Prepare a wash solution of sufficient volume to add 30 mL to every
ThinPrep Pap test specimen being reprocessed. The wash solution is
made by mixing 9 parts CytoLyt Solution with 1 part glacial acetic
acid.
2 Prior to performing this step, assure there is sufficient volume in the
ThinPrep Pap test specimen to result in a pellet, following centrifu-
gation. Pour the contents of the ThinPrep Pap test specimen into a
centrifuge tube appropriately labeled to maintain chain of custody.
Retain the vial.
3 Pellet the contents of the centrifuge tube by centrifugation at 1200 x
g
for 5 minutes.
Note:
Once centrifugation is complete, the cell pellet should be
clearly visible but the cells may not be tightly packed
together (the pellet may appear fluffy).
4 a. Carefully pour off the supernatant from the centrifuge tube to
avoid loss of cells. Dispose of according to local regulations.
b. Vortex the centrifuge tube briefly.
c. Pour 30 mL of the CytoLyt Solution and 10% glacial acetic acid
mixture into the centrifuge tube and cap securely.
d. Invert the centrifuge tube by hand several times to mix.
5 Pellet the cells again by centrifugation — 1200 x
g
for 5 minutes.
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