Hologic thinprep 2000 - SECTION D-8: Mechanical Agitation

NON-GYNECOLOGIC SAMPLE PREPARATION
ThinPrep 2000 Processor Operator’s Manual 1.15
1
MECHANICAL AGITATION
Mucoid specimens require vigorous agitation in CytoLyt Solution to break up the mucus. Hologic
recommends two methods of mechanical agitation:
Method A:
Vortex the CytoLyt/sample mixture for at least 5 minutes on a “hands-free” vor-
texor. The vortexor speed must be adjusted to produce visible agitation to the
bottom of the tube.
Method B:
Blend the CytoLyt/sample mixture for a few seconds.
Note: Agitation times for both methods may vary due to differences in specimen consistency.
The blending technique may show fragmentation or disruption of cell architecture. Excessive
blending must be avoided.
Vortexing for at least 5 minutes after blending helps break up more mucus.
SECTION
D-8
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