NON-GYNECOLOGIC SAMPLE PREPARATION
1.28
ThinPrep 2000 Processor Operator’s Manual
Bloody or Proteinaceous Specimens
Problem Procedure
A. Did the “Sample Is
Dilute” message
appear during process-
ing?
1. Check to see if cellularity is adequate. If
not, use more of the pellet if available.
Prepare a slide using Sequence 2.
B. Does the slide have an
obvious “halo” of cel-
lular material and/or
white blood cells?
1. Dilute the sample 20:1. Use a calibrated
pipette to add 1 mL of sample to a new
PreservCyt Solution vial. Prepare slide
using Sequence 1.
If a halo is present on the new slide, call
Hologic Technical Service.
C. Is the slide sparse and
does it contain blood,
protein, or non-cellular
debris?
1. Pour the contents of the PreservCyt Sam-
ple vial into a centrifuge tube.
Call Hologic Technical
Service.
2. Concentrate by centrifugation — 600g x
10 min.
3. Pour off supernatant and vortex to
resuspend cell pellet.
4. If the sample contains blood or non-cel-
lular debris:
Mix a solution of 9 parts CytoLyt Solu-
tion to 1 part glacial acetic acid.
Add 30 mL of this solution to the sample
centrifuge tube.
If the sample contains protein:
Add 30 mL of saline to the sample cen-
trifuge tube.
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