FIXATION, STAINING, AND COVERSLIPPING
ThinPrep 2000 Processor Operator’s Manual
8.3
STAINING
General guidelines to consider when staining ThinPrep slides are:
• Staining times may be different and may require adjustment for ThinPrep slides compared to
conventional preparations.
• The use of graded concentrations of alcohol in the staining process will minimize cell distor-
tion and possible cell shedding.
• The use of mild bluing solutions and dilute acid baths will optimize nuclear staining and min-
imize possible cell shedding.
Staining Protocol:
A recommended staining protocol for ThinPrep slides is attached. This protocol incorporates the
general staining guidelines stated above and the following specific recommendations:
1. If slides have been spray fixed, remove the spray fixative by soaking in a standard laboratory fix-
ative for at least 10 minutes.
2. Stain the ThinPrep slides with standard modified Papanicolaou stains according to the manufac-
turer’s routine procedures adjusting to the general guidelines for ThinPrep slide staining stated
above.
3. Standard staining times for ThinPrep slides may be different from conventional slides, and it
may be necessary to increase or decrease these times. It is recommended that staining times be
optimized following laboratory standard operating procedures. These differences may necessi-
tate staining ThinPrep and conventional slides separately.
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