INTRODUCTION
1.8
ThinPrep 2000 Processor Operator’s Manual
Sample Preparation/Instrument Loading
Before the ThinPrep processor can process gynecologic samples, the samples must be placed into
PreservCyt Solution. Gynecologic samples must be prepared according to the protocols described in
Chapter 4, “Gynecologic Sample Preparation”. Once the cells are added to the PreservCyt Solution
vial by the appropriate method, the instrument can process the sample vial.
In preparation for sample processing, the operator loads four essential items into the ThinPrep 2000
processor: a PreservCyt Sample vial, a ThinPrep Pap test filter attached to the filter cap, a ThinPrep
slide and a fixative vial containing a standard laboratory fixative. The processes of loading and
operating the instrument are explained in Chapter 5A, “Operating Instructions”.
Start of Cycle
When the operator initiates a sequence, the ThinPrep 2000 processor verifies installation of
disposables, motor positions, and the positive and negative pressures in the pressure reservoirs.
After this the instrument processes the slide using the selected sequence.
Fluid Level Detection
The cap seal lowers to seal the filter assembly and the sample vial is raised towards the filter
membrane. The sample vial stops when the filter membrane makes contact with the surface of fluid.
If the fluid level is satisfactory, the instrument will continue the slide preparation process. An error
message and audible alarm indicate an unsatisfactory fluid level.
Dispersion
The cap seal lifts and the dispersion system rotates the ThinPrep Pap test filter assembly within the
cell suspension, creating shear forces in the fluid that are strong enough to separate randomly joined
material and disperse mucus, and are not known to have an adverse effect on the cellular
architecture or on adhesive forces joining diagnostically relevant groups of cells.
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