GYNECOLOGIC SAMPLE PREPARATION
ThinPrep 2000 Processor Operator’s Manual
4.5
SPECIAL PRECAUTIONS
PreservCyt Solution
For more information on PreservCyt Solution, refer to Chapter 3, “PreservCyt Solution”
.
Interfering Substances
The Clinical and Laboratory Standard Institute Guidelines (formerly NCCLS) recommend
that no lubricant be used during Pap testing.
1
ACOG recommends that care be taken not to contaminate the specimen with lubricant
because this may lead to unsatisfactory results.
2
This applies to both conventional Pap testing
and liquid-based cytology.
If you are using a plastic speculum, or in instances where a lubricant must be used, take care
not to contaminate the cervix or collection devices with the lubricant. A tiny amount of lubri-
cant may be used, just enough to sparingly coat the speculum with a gloved finger, avoiding
the tip of the speculum.
The Clinical and Laboratory Standard Institute Guidelines and ACOG recommend that you
not take a Pap during menses.
1
-
2
For samples to be processed on the ThinPrep 2000 processor, lubricants can adhere to the fil-
ter membrane and may cause poor cell transfer to the slide. If its use is unavoidable, the
lubricant should be used in minimum amounts.
After sample transfer to the PreservCyt Solution vial, the sample
should stand for at least 15 minutes before processing.
1.
Papanicolaou Technique Approved Guidelines (CLSI Document GP15-A3,
third edition, 2008)
2.
ACOG Practice Bulletin, no. 45, August 2003
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