Hologic thinprep 2000 - Hologic Staining Protocol

FIXATION, STAINING, AND COVERSLIPPING
ThinPrep 2000 Processor Operator’s Manual 8.5
8
Table 8.1: Hologic Staining Protocol
Stains may be ordered from Richard-Allan Scientific, SC .
Hematoxylin I (7221), Richard-Allan Cytology Stain (7511). Permount is a trademark of Fisher
Scientific Corp.
* Time may vary with stain lot or age.
Solution Time
1. 70% Reagent Alcohol 1 minute with agitation
2. 50% Reagent Alcohol 1 minute with agitation
3. Distilled H
2
O (dH
2
O) 1 minute with agitation
4. Richard-Allan Hematoxylin I 30 seconds with agitation*
5. Distilled H
2
O (dH
2
O)) 15 seconds with agitation
6. Distilled H
2
O (dH
2
O)) 15 seconds with agitation
7. Clarifier (0.025% glacial acetic acid) 30 seconds with agitation
8. Distilled H
2
O (dH
2
O)) 30 seconds with agitation
9. Bluing Reagent (10 mg LiCarb/1 L) 30 seconds with agitation
10. 50% Reagent Alcohol 30 seconds with agitation
11. 95% Reagent Alcohol 30 seconds with agitation
12. Richard-Allan Cytology Stain 1 minute with agitation
13. 95% Reagent Alcohol 30 seconds with agitation
14. 95% Reagent Alcohol 30 seconds with agitation
15. 100% Reagent Alcohol 30 seconds with agitation
16. 100% Reagent Alcohol 30 seconds with agitation
17. 100% Reagent Alcohol 30 seconds with agitation
18. Xylene 1 minute with agitation
19. Xylene 1 minute with agitation
20. Xylene 3 minutes with agitation
21. Mount in Permount