NON-GYNECOLOGIC SAMPLE PREPARATION
ThinPrep 2000 Processor Operator’s Manual
1.33
Techniques Used in Troubleshooting
Diluting the Sample 20 to 1
To dilute a sample suspended in PreservCyt Solution, add 1 mL of the sample that is suspended in
PreservCyt Solution to a new PreservCyt Solution Vial (20 mL). This is most accurately done with a
calibrated pipette.
You may also simply count drops from an uncalibrated plastic pipette if you know how many drops
correspond to 1 mL. To calculate this, count out drops of PreservCyt Solution into a container of
known volume. When the known volume is reached, divide the number of drops by the volume (in
ml) to get the number of drops that corresponds to 1 mL. Use PreservCyt Solution rather than any
other liquid so the drop size will be consistent with the sample drops.
Glacial Acetic Acid Wash for Blood and Non-Cellular Debris
If a sample is found to be bloody during microscopic review, it can be further washed using a solu-
tion of 9 parts CytoLyt Solution and 1 part glacial acetic acid. This should only be done after the
sample has been in PreservCyt Solution. Do not use directly with fresh specimens; nuclear morphol-
ogy may not be adequately preserved.
Saline Wash for Protein
If a sample is found to contain protein during microscopic review, it can be further washed using
saline solution in place of CytoLyt Solution. This should only be done after the sample has been in
PreservCyt Solution. Do not use directly with fresh specimens; nuclear morphology may not be ade-
quately preserved.
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