Hologic thinprep 2000 - SECTION E-4: Firstcyte Breast Test Specimens

NON-GYNECOLOGIC SAMPLE PREPARATION
ThinPrep 2000 Processor Operator’s Manual 1.23
1
FIRSTCYTE
®
BREAST TEST SPECIMENS
Specimens collected via FirstCyte breast test.
1. Collection: Collect sample directly into 30 mL of CytoLyt Solution in
the CytoLyt tube.
2. Concentrate by Centrifugation — 600g for 10 minutes.
3. Pour off supernatant and resuspend cell pellet.
Resuspension can be done on a vortexor or may be achieved by
syringing the pellet back and forth with a plastic pipette.
4. Add PreservCyt Solution from a PreservCyt Solution vial. Cap the
tube tightly and mix by inversion to resuspend all the cells.
5. Add appropriate amount of specimen to PreservCyt Solution Vial.
Refer to page 1.12.
Allow to stand in PreservCyt Solution for 15 minutes.
6. Run on ThinPrep 2000 processor using
Sequence 2 , Fix, Stain and
Evaluate.
SECTION
E-5
ThinPr
ep