NON-GYNECOLOGIC SAMPLE PREPARATION
1.10
ThinPrep 2000 Processor Operator’s Manual
POUR OFF SUPERNATANT AND VORTEX TO RESUSPEND CELL PELLET
Pour off the supernatant completely to effectively concentrate the sample. To do
this, invert the centrifuge tube 180 degrees in one smooth movement, pour off all
the supernatant, and then return the tube to its original position as shown in Fig-
ure 1-3
1
.
Observe the cell pellet during inversion to avoid accidental loss of
cellular material.
Caution:
Failure to completely pour off the supernatant may produce a sparse sample and an
unsatisfactory slide due to dilution of the cell pellet.
Figure 1-3 Pouring Off Supernatant
After pouring off the supernatant, place the centrifuge tube onto a vortexor and agitate the cell pellet
for 3 seconds. Manual vortexing may be achieved by syringing the pellet back and forth with a plas-
tic pipette. The intention of this vortexing step is to randomize the cell pellet before transferring to
the PreservCyt Solution vial and to improve the results of the CytoLyt Solution washing procedure.
1 Refer to Bales, CE, and Durfee, GR. Cytologic Techniques in Koss, L. ed. Diagnostic Cytology and
its Histopathologic Basis. 3rd Edition. Philadelphia: JB Lippincott. Vol. II: pp. 1187–12600 for
details.
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