NON-GYNECOLOGIC SAMPLE PREPARATION
1.2
ThinPrep 2000 Processor Operator’s Manual
CONTENTS
This chapter is divided into the following five main sections and several subsections:
SECTION C: Required Materials
1.3
SECTION D: Details of Non-gynecologic Sample
Preparation Steps
1.4
SECTION D-1:
Collection 1.5
SECTION D-2:
Concentrate
by Centrifugation — 600g for 10 min 1.8
SECTION D-3:
Pour Off Supernatant and
Vortex to Resuspend Cell Pellet 1.10
SECTION D-4:
Evaluate Cell Pellet Appearance 1.11
SECTION D-5:
Add Specimen to
PreservCyt
®
Solution Vial 1.12
SECTION D-6:
Allow to Stand in PreservCyt Solution
for 15 Min 1.13
SECTION D-7:
Run on ThinPrep
®
2000 Processor Using
Sequence n, Fix, Stain, and Evaluate 1.14
SECTION D-8:
Mechanical Agitation 1.15
SECTION D-9:
CytoLyt
®
Solution Wash 1.16
SECTION E: Specimen Preparation Protocols
1.17
SECTION E-1:
Fine Needle Aspirates 1.18
SECTION E-2:
Mucoid Specimens 1.19
SECTION E-3:
Body Fluids 1.21
SECTION E-4:
Superficial Brushings And Scrapings 1.22
SECTION E-4:
FirstCyte
®
Breast Test Specimens 1.23
SECTION F: ThinPrep
®
UroCyte
®
Specimens
1.24
SECTION G: Sample Preparation Troubleshooting
1.27
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