134 Agilent 7100 Capillary Electrophoresis System User Manual
5 Using the Agilent 7100 Capillary Electrophoresis System
Capillary Electrophoresis, CE
6 Move the cassette guide back again while holding the cassette down. The
alignment interface aligns the capillary automatically with the detector.
7 Close the top cover and secure it by latching it onto the body of the case.
For safety reasons, no power is applied if the top cover is open.
Because the cassette was changed, the Agilent ChemStation automatically
performs the wavelength calibration in the background. It tests the
wavelength calibration of your detector and advises you to make
adjustments, if necessary.
If the lamp is off or the cassette is not inserted properly, the wavelength
cannot be adjusted correctly.
8 Close the Change Cassette dialog box by choosing OK, enter the capillary
information and proceed to setting up a method.
One of the following not ready conditions may appear in the status display of
the Agilent ChemStation. Move the mouse over the status display to read the
not ready conditions:
• Recalibration necessary
• UV lamp not ready
To correct for these not ready conditions please reinsert the cassette. Make
sure the alignment interface snaps onto the ceramic interface of the detector
and is not occluded. Make sure the lamp is ignited.
Only alignment interfaces dedicated to the 7100 CE instrument will fit. Interfaces from
different instruments will not fit and will lead to unsatisfactory results.
Damage to the cassette
Closing the top cover with an improperly installed cassette may damage the cassette.
• Do not close the top cover, if the cassette is not properly installed.