Vials and Bottles 6
Installation, Operation, and Maintenance 111
Part 2, Operation
Selecting vial septa
There are two types of septa used with crimp caps and screw-on caps, each
with different resealing characteristics and different resistance to solvents.
• One type is natural rubber formulation coated with Teflon on the sample
side. This septum is suitable for samples with a pH range of 4.0 to 7.5. They
are less resistant to solvents after puncture and are more easily cored than
silicone rubber septa. Coring may deposit septum pieces in the vial and
affect your chromatograms.
• Another is high-quality, low-extractable silicone rubber septa, coated with
Teflon on one or both sides. They are more resistant to solvents after
puncture and to coring by the needle.
Refer to your Agilent catalog for consumables and supplies for more
information.
Figure 45 shows the diameter for vial cap apertures.
Figure 45 Vial cap aperture specifications
5.58 mm (0.220 in.) recommended
4.65 mm (0.183 in.) minimum