130 Installation, Operation, and Maintenance
Part 2, Operation
7 Running Samples
Running a Sample
To operate your automatic liquid sampler:
1 Install a clean syringe. See “Installing a Syringe" on page 103.
2 Fill the solvent bottles. See “Preparing Solvent and Waste Bottles" on
page 116.
3 Place the solvent and waste bottles in the turret. See “Placing Vials and
Bottles in the Sampler" on page 118.
4 Load the sample vials into the turret or the tray. See “Preparing Sample
Vials" on page 110.
5 Prepare the GC sequence. See your GC or ChemStation documents.
6 Run the sequence by pressing the start button on the GC. On a 5890 GC you
may optionally press the start button on the ALS controller. When the GC is
ready, the ALS begins the injections.
Injection volume
The injection volume depends on the sample size and the syringe size.
• Syringe size may be 1 µL, 5 µL, 10 µL, 25 µL, 50 µL, or 100 µL.
• Sample size may be 2%, 10%, 20%, 30%, 40%, or 50% of the syringe size.
After syringe size is selected, sample sizes are shown in microliters (Table 21).
WARNING
When running a sample, keep your hands away from the syringe needle. The needle
is sharp and may contain hazardous chemicals.
Table 2 1 Syringe and sample sizes
Syringe size, µL Available sample sizes, µL
2%
*
10% 20% 30% 40% 50%
* Nanoliter adapt must be set to on for 6890 GC.
1 0.02 0.1 0.2 0.3 0.4 0.5
5 0.1 0.5 1.0 1.5 2.0 2.5
10 0.2 1.0 2.0 3.0 4.0 5.0
25 0.5 2.5 5.0 7.5 10.0 12.5
50 1.0 5.0 10 15 20 25
100 2.0 10 20 30 40 50
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