Syringes and Needles 5
Installation, Operation, and Maintenance 107
Part 2, Operation
Replacing Syringe Needles
The stainless steel needles used for 250-µm and 320-µm injections must be
inserted into a glass syringe barrel (the 5-µL syringe barrel is
part no. 5182–0836). Select the correct size needle for the column you will use.
Needles for 250-µm injections (part no. 5182-0833, 3/pk) have silver-colored
stops. Needles for 320-µm injections (part no. 5182-0831, 3/pk) have
gold-colored stops. See your Agilent catalog for consumables and supplies for
a complete list of syringes and needles.
To insert a needle into a syringe barrel (Figure 43):
1 Unscrew the syringe barrel cap and remove the spring.
2 Make sure the needle has the Teflon® disk as shown in Figure 43. If the
syringe barrel does not have the Teflon® disk, use the instructions in the
syringe box to wrap the needle yourself.
3 Slide the spring and the cap down over the needle.
4 Insert the needle into the syringe barrel.
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