4-4
P129394-176 Operator Manual Techniques of Sterilization
4.2 Immediate Use Each of the sterilizers is programmed with one or more sterilization
cycles intended to process items for “immediate use.” Immediate
use can be defined as the shortest possible time between a
sterilized item’s removal from the sterilizer and its aseptic transfer to
the sterile field.
• A sterilized item intended for immediate use must not be stored.
• An item sterilized for immediate use cannot be held for use on a
future case.
• The Prevac immediate use cycle is the preferred immediate use
cycle to use. The Gravity immediate use cycle is only safe for
simple instruments that contain no hinges or other features that
could trap air.
4.3 Control Measures
for Verifying
Sterilization Process
A live spore test utilizing B. stearothermophilus is the most reliable
form of biological monitoring. This type of product utilizes controlled
populations of a controlled resistance, so that survival time and kill
time can be demonstrated.
To verify the process, insert the biological indicator in a test pack
and place pack on the bottom shelf. Run test pack through a typical
cycle. On completion, forward test pack and monitor to appropriate
personnel for evaluation. Refer to AAMI guidelines to conduct routine
biological monitoring.
Gravity Cycle 270°F
(132°C)
25 Minutes 15 Minutes Fabric Packs ST-8:2008
Immediate Use
Gravity Cycle
270°F
(132°C)
3 Minutes 1 Minute Unwrapped instrument tray containing
a single instrument; or multiple
unwrapped instrument trays, non-
porous load. Maximum weight per
tray, 25 lb (11.3 kg)
ST-8:2008
Immediate Use
Gravity Cycle
270°F
(132°C)
10 Minutes 1 Minute ST-8:2008
Liquid Cycle 250°F
(121°C)
45 Minutes N/A Refer to Table 5 (page iv) for
recommended quantities
ST-8:2008
6. Leak Test
**
N/A N/A N/A None ST-8:2008
7. Dart Test** 270°F
(132°C)
3-1/2
Minutes
1 Minute Dart or Bowie-Dick Test Pack ST-8:2008
8. Dart Warm-up** 270°F
(132°C)
3 Minutes 1 Minute None N/A
* Dry time can be reduced to 5 minutes for a single fabric pack.
** Not adjustable.
Table 4-2. AMSCO C Series SFPP Sterilizer Cycles (Continued)
Cycles:
Sterilize
Temp.
Sterilize
Time
Dry
Time
Recommended Load
Refer to Table 4 (page iii) for
Recommended Quantities
Validation
Standard