CEM liberty - Introduction to Pepdriver

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
20
Introduction to PepDriver
The operation of the Liberty is controlled through the PepDriver software package. The Liberty
includes an external computer controller (either a laptop or desktop Windows PC) for running
PepDriver. This computer is connected to the Liberty and the Discover through an ethernet
connection.
PepDriver Terminology
Throughout this manual, specic terms will be used to describe the various functions within
PepDriver.
• Microwave Method: The specic parameters used in a Microwave step
within a cycle. Microwave Methods can be created or modied using the
Microwave Editor (p. 25).
• Cycle: The specic steps used for each residue within a given sequence.
Cycles can be created or modied using the Cycle Editor (p. 28).
• Sequence: The specic peptide to be synthesized in a given method.
Sequences can be created or modied using the Sequence Editor (p. 32).
• Method: The specic parameters used to synthesize a peptide. For each
method, a sequence is selected, and then parameters (individual coupling
cycles for each residue, C-terminus type, nal deprotection, etc.) are also
selected as part of the method. Methods can be created or modied using
the Method Editor (p. 36).
• Run: A specic instance of a method being loaded and started in PepDriver.
A Run History le (p. 56) is recorded for each run.