Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
43
Cleavage Calculator
The Cleavage Calculator allows for the custom calculation of each scavenger and additive for
whatever cocktail will be best for a given peptide. To calculate how to make a cleavage cocktail:
Enter the total volume required for the method in the Total Volume box at the top 1.
of the calculator.
Click on the rst reagent to be used. Enter the desired nal percent of that 2.
reagent in the Percent box on the right.
The percent and volume of 3. TFA required will automatically be updated. Repeat
step 2 for the remaining scavengers.
The calculator will automatically update the amounts required for each reagent 4.
as changes are made. When all of the percentages are correct, the required
amount of each reagent to be added will be displayed in grams (for solids) and
mL (for liquids).
NOTE
CEM recommends using 95% TFA, 2.5% TIS, 2.5% DODT, and 2.5% water
as the cleavage cocktail.
To Next Page
Loading...
