CEM liberty - Semiannual Maintenance; Standby Procedure

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
62
Verify the performance of the • ltered drain by adding 10 mL of DMF to the
reaction vessel and then performing a ltered drain. The reaction vessel
should drain 10 mL of DMF within 10 seconds. If the drain takes longer than 10
seconds, replace the F3 lter and try again. If the drain still takes longer than 10
seconds, replace the reaction vessel.
Inspect the reaction vessel for particulate accumulation, particularly on the top •
of the vessel. If necessary, clean the reaction vessel (from the Maintenance
Screen, on the Cleaning tab, click the Clean RV button).
Review recent • run history reports (see Run History Reports, p. 59, for details)
to ensure that the Liberty is heating properly. The temperature for a coupling
should reach 75 °C within 70 seconds. If the Liberty is not reaching temperature
in time, increase the power setting in the Microwave Editor (see Editing an
Existing Microwave Method, p. 29 for details). If the Liberty is routinely heating to
above 83 °C, decrease the power setting in the Microwave Editor.
Semiannual Maintenance
Replace the reaction vessel body.•
Verify the proper operation of the waste sensor.•
Standby Procedure
If the Liberty will be idle for a period of two weeks or more:
Remove all reagents (amino acids, activator, activator base, deprotection, •
capping, cleavage) from the system.
Backush all positions except Main Wash/DMF and Secondary Wash/DCM.•
Remove the wash solvents and perform a backpurge on those positions to empty •
the lines.
Prior to using the Liberty after it has been in Standby:
Connect the wash solvents using the • Change Bottle command (p. 65) to properly
prime the lines and pressurize the bottles.
Perform a backush on all positions except Main Wash/DMF and Secondary •
Wash/DCM. (See Performing a Backush, p. 66, for details)
Perform a • Sensor Calibration on all sensors. (See Performing a Sensor
Calibration, p. 76, for details.)
Perform a • Volume Calibration for all additions except TFA. (See Performing a
Volume Calibration, p. 70, for details.)