Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
46
Preparing Activator Solution
Weigh out the appropriate amount of the desired activator (as calculated using 1.
the Usage and Reagent Calculators) and transfer to a 250 mL amber glass
bottle.
Add the appropriate volume of DMF.2.
Put a cap on the bottle. Gently swirl the bottle to ensure that all of the activator 3.
has gone into solution.
Preparing Activator Base Solution
Measure out the appropriate amount of activator base and transfer to a clear 250 1.
mL glass bottle.
Add the appropriate volume of NMP to give the correct nal volume.2.
Put a cap on the bottle. Gently swirl the bottle to ensure all of the activator base 3.
has gone into solution.
Preparing Amino Acids
CEM offers a full line of pre-weighed amino acids in 5 mmol, 10 mmol, and 20 mmol sizes. It is
not required to use the pre-weighed amino acids.
To use CEM pre-weighed amino acids:
Open the bottles, breaking the safety seals.1.
Add the appropriate amount of DMF to each bottle (25 mL for 5 mmol bottles, 50 2.
mL for 10 mmol bottles, and 100 mL for 20 mmol bottles).
Replace the caps and shake vigorously to ensure all amino acid has gone into 3.
solution.
NOTE
Some activators, especially the aminium activators such as HBTU, are light
sensitive and should be prepared in an amber glass bottle.
NOTE
Some activators, notably HBTU, may take up to 10 minutes to fully go into
solution.
NOTE
CEM recommends using DIEA as the activator base. When using DIEA,
NMP must be used as the solvent because DIEA is not miscible in DMF at
the standard concentration of 2 M. The nal concentration of DIEA in the
reaction vessel is low enough that miscibility is not an issue.
NOTE
Amino acid, activator, activator base, and deprotection solutions can be
used for up to two weeks. Some amino acids, notably His, will begin to
crash out of solution after two weeks.
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