CEM liberty - Modifying a Sequence; Using the External Amino Acid Positions

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
33
Modifying a Sequence
If a sequence is entered incorrectly, there are a few ways to correct the error.
To insert an amino acid:
Right-click on the amino acid that will come after the inserted amino acid.1.
Select Insert Amino Acid.2.
A box will pop up stating that the next amino acid entered will be inserted before 3.
the current amino acid. Click OK to close the box.
Click on the desired amino acid button or type the one letter abbreviation of the 4.
desired amino acid.
To delete an amino acid:
Right-click on the amino acid.1.
Select Remove Amino Acid.2.
To move an amino acid within a sequence:
Click on the amino acid to be moved.1.
Click the Move Left (<-Move) or Move Right (Move->) buttons at the bottom of 2.
the Editor window to move the amino acid one position in the indicated direction.
Repeat as needed until the amino acid is in the correct position.
Using the External Amino Acid Positions
There are ve external positions amino acid positions, labeled in the Sequence Editor as EX1
through EX5. The external amino acids can be used in a sequence by either clicking the
corresponding button or by typing the number of the external position into the sequence.
By default, the molecular weight of the external amino acids will not be included in the sequence
molecular weight calculation. To enable an external amino acid weight:
Click the MW box next to the appropriate external amino acid button. This will 1.
open the Amino Acid Weight conguration window for that position.
Check the Enable Ext Amino Acid Weights box.2.
Enter the protected and unprotected molecular weights for the amino acid being 3.
used.
Click OK. The Amino Acid Weight conguration window will close, and the 4.
unprotected weight will appear in the MW box next to the button.