CEM liberty - Section 3: Quick Setup Guide; Peptide Setup; Create and Load the Method

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
57
Section 3: Quick Setup Guide
Liberty Setup
Create and Load the Method
1. Create a new sequence.
Open 1.1. Sequence Editor by clicking on the Sequence button at the top.
Click on the folder where the sequence should be saved, then click the 1.2.
New Sequence button.
Enter a name for the sequence and press Enter.1.3.
Enter the sequence either by clicking on the amino acid buttons or by 1.4.
typing the one letter abbreviations for the amino acids into the Sequence
box.
Click Save, then close the Sequence Editor.1.5.
2. Create a new method.
Open the 2.1. Method Editor by clicking on the Methods button.
Click on the folder where the method should be saved, then click New 2.2.
Method.
Enter a name for the method and press Enter.2.3.
Choose the desired sequence from the appropriate folder in the 2.4.
Sequences window. The selected sequence will be loaded into the
Sequence box.
Select all method parameters:2.5.
Resin Information: 2.5.1.
2.5.1.1. Scale: Select the scale for the synthesis of the peptide (0.05
mmol to 5.0 mmol).
NOTE
The amino acids in the sequence should be entered from N-terminus to
C-terminus.
CAUTION
The 125 mL reaction vessel must be used for syntheses above 0.25 mmol.
The 10 mL reaction vessel should be used for syntheses below 0.1 mmol.