CEM liberty - Cycle Types; Creating a Cycle

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
29
PepDriver comes with optimized default cycles for resin transfer, amino acid addition, nal
deprotection, and cleavage for each of the available synthetic scales.
Cycle Types
• Resin: Resin cycles control how resin is transferred from the resin position into
the reaction vessel at the beginning of a synthesis, as well as the resin swelling
step.
• Amino Acid: Amino acid cycles control how the deprotection and coupling steps
of one amino acid in the sequence occur.
• Cleaving: Cleaving cycles control if and how the peptide is removed from the
resin following synthesis, including the return of either cleaved peptide to the
product position or peptide on resin to the resin position.
• Final Deprotection: Final deprotection cycles control the removal of the
N-terminal protecting group, and also allow for N-terminal acetylation of the
peptide before cleavage.
Creating a Cycle
Cycles allow for detailed control of each action taken in a given step of a peptide synthesis run.
PepDriver comes with default cycles of each type for each synthetic scale; however, cycles can
be customized to accomplish a number of non-standard chemistries. To create a new cycle:
Click the New Cycle button at the top of the Cycle Editor window and select the 1.
type of cycle to be created. For Amino Acid, Cleaving, and Final Deprotection the
synthesis scale must be selected.
The cycle will appear in the appropriate folder. Enter a name for the cycle and 2.
press Enter.
Step 1 will appear in the cycle window. 3.
Click the 4. Operation box and select the operation to be performed. (For a full list
of available operations, see Appendix 3: Operations in the Cycle Editor.)
NOTE
A cycle saved in a specic scale folder will only be available for use in
Method Editor for methods created at that scale.