CEM liberty - Reagent, Amino Acid, and External Amino Acid Addition

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
93
and the specic sensor that failed will be recorded in the status log. If any
sensors fail, contact CEM Service for troubleshooting help.
Reagent, Amino Acid, and External Amino Acid Addition
There are three types of addition tests available in Test System: Reagent, Amino Acid, and
External Amino Acid. The addition tests are intended to verify that the sample loops for each of
these positions are delivering the correct volume.
To perform an Addition Test:
Disconnect the sprayhead line from the manifold on the front of the Liberty. 1.
Connect a piece of 1/8” OD tubing (PN BR199116) to the manifold using a nut
(PN 167810) and yellow ferrule (PN 164315).
Place the open end of the tubing into a 10 mL graduated cylinder.
Select the category of addition test, and then double-click on the individual 2.
position to be tested in the Test window (or click Test to test all additions within
the category).
The Liberty will begin adding one sample loop of the selected reagent. The 3.
progress of the addition and the target volume will be displayed in the status
window.
When the addition is complete, verify the volume delivered to the graduated 4.
cylinder. If the delivered volume is more than 0.3 mL different from the target,
contact CEM Service for troubleshooting assistance.