CEM liberty - Section 3: Quick Setup Guide; Initial Liberty Setup; Method Creation and Loading

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
55
Sample Method Report
None
Method Comments:
0.10-Cleavage
Cleaving:
4/23/2005 4:41:29 PM
Modified Date:
Yes
Resin Load:
0.10
Final Deprotection:
Acid
C - Terminus:
0.6
Substitution:
0.10
Scale:
Sequence:
V Q A A I D Y I N G
1063.17Total MW:
ACP
Method
Resin
Cycles
Resin:
Standard - Resin
Amino Acid:
Specific Deriative:
Cycle:
Amino Acids
Gly - G
Standard - Resin
Fmoc-Gly-OH
Asn - N
0.10-Single
Fmoc-Asn(Trt)-OH
Ile - I
0.10-Single
Fmoc-Ile-OH
Tyr - Y
0.10-Single
Fmoc-Tyr(tBu)-OH
Asp - D
0.10-Single
Fmoc-Asp(OtBu)-OH
Ile - I
0.10-Single
Fmoc-Ile-OH
Ala - A
0.10-Single
Fmoc-Ala-OH • H
2
O
Ala - A
0.10-Single
Fmoc-Ala-OH • H
2
O
Gln - Q
0.10-Single
Fmoc-Gln(Trt)-OH
Val - V
0.10-Single
Fmoc-Val-OH
Resin Cycle - Standard - Resin
No
Operation
Parameter
Volume(mL) Drain Cycles Pause
1
Clean Reaction Vessel - Unfiltered
10.0
Yes
1
No
2
Add DMF to Resin
7.0
No
1
No
3
Add DCM to Resin
7.0
No
1
No
4
Transfer Resin to Reaction Vessel
10.0
Yes
1
No
5
Add DMF to Resin
7.0
No
1
No
6
Add DCM to Resin
7.0
No
1
No
7
Transfer Resin to Reaction Vessel
10.0
Yes
1
No
8
Add DMF to Resin
7.0
No
1
No
9
Add DCM to Resin
7.0
No
1
No
10
Transfer Resin to Reaction Vessel
10.0
Yes
1
No
11
Add Reagent
DMF
5.0
No
1
No
12
Add Reagent
DCM
5.0
No
1
No
13
Wait State
900 secs
No
1
No
14
Drain - Filtered
Yes
1
No
Amino Acid Cycle - 0.10-Single
No
Operation
Parameter
Volume(mL) Drain Cycles Pause
1
Clean Resin Dip Tube
Yes
1
No
2
Wash - Top
DMF
7.0
Yes
1
No
3
Add Deprotection
7.0
No
1
No
4
Microwave Method
Initial Deprotection
Yes
1
No
5
Wash - Top
DMF
5.0
Yes
1
No
6
Add Deprotection
7.0
No
1
No
7
Microwave Method
Deprotection
Yes
1
No
8
Wash - Top
DMF
7.0
Yes
1
No
9
Wash - Bottom
DMF
7.0
Yes
1
No
10
Wash - Top
DMF
7.0
Yes
1
No
11
Clean Resin Dip Tube
Yes
1
No
12
Wash - Top
DMF
7.0
Yes
1
No
13
Add Amino Acid
2.5
No
1
No
14
Add Activator
1.0
No
1
No
15
Add Activator Base
0.5
No
1
No
16
Microwave Method
Coupling
Yes
1
No
17
Wash - Top
DMF
7.0
Yes
1
No
18
Wash - Bottom
DMF
7.0
Yes
1
No
19
Wash - Top
DMF
7.0
Yes
1
No
Final Deprotect Cycle - 0.10
No
Operation
Parameter
Volume(mL) Drain Cycles Pause
1
Clean Resin Dip Tube
Yes
1
No
2
Wash - Top
DMF
7.0
Yes
1
No
3
Add Deprotection
7.0
No
1
No
4
Microwave Method
Initial Deprotection
Yes
1
No
5
Clean Resin Dip Tube
Yes
1
No
6
Add Deprotection
7.0
No
1
No
7
Microwave Method
Deprotection
Yes
1
No
8
Wash - Top
DMF
7.0
Yes
1
No
9
Wash - Bottom
DMF
7.0
Yes
1
No
10
Wash - Top
DMF
7.0
Yes
1
No
Cleavage Cycle - 0.10-Cleavage
No
Operation
Parameter
Volume(mL) Drain Cycles Pause
1
Wash - Top
DCM
7.0
Yes
4
No
2
Wash - Bottom
DCM
7.0
Yes
1
No
3
Clean Resin Dip Tube
Yes
1
No
4
Wash - Top
DCM
7.0
Yes
1
No
5
Add Cleavage (TFA) - Sample Loop
8.0
No
1
No
6
Microwave Method
Cleaving 35min
No
1
No
7
Transfer Product Cleaved
No
1
No
8
Add Reagent
DCM
5.0
No
1
No
9
Transfer Product Cleaved
No
1
No
10
Add Reagent
DCM
5.0
No
1
No
11
Transfer Product Cleaved
No
1
No
12
Clean Reaction Vessel - Unfiltered
10.0
No
3
No
13
Reaction Vessel Neutralization
10.0
No
3
No
14
Wash - Top
DCM
7.0
Yes
2
No