CEM liberty - Running the Liberty; Preparing the Liberty to Run

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
51
Running the Liberty
Preparing the Liberty to Run
Verify the level of the waste container to ensure there is sufcient room before 1.
starting another synthesis.
Verify the nitrogen supply to ensure there is sufcient nitrogen to complete the 2.
synthesis.
Verify that all amino acids required for loaded methods are loaded in the 3.
appropriate positions.
Verify that the resins are loaded into the positions corresponding to the correct 4.
methods in PepDriver, and that tubes are connected to the corresponding
product positions if cleaving.
Verify the levels of both wash solvents (DMF and DCM) to ensure there is 5.
sufcient solvent.
Verify that all sequences and methods are correct and that they are loaded in the 6.
correct positions.
Verify that the appropriate reaction vessel for the scale of the loaded methods is 7.
connected, and that the ber optic probe is fully inserted into the thermowell.
Verify that the method is correct by looking at the steps outlined in the Method 8.
box. If there are any errors, make corrections and reload the method.
Right-click on the green resin position where the method was loaded and 8.1.
select Clear Method.
To correct the method:8.2.
Open the Method Editor. In the Methods box on the left, open the 8.2.1.
folder where the method was saved. Click on the method to load it.
Make any corrections as needed (see p. 38). 8.2.2.
Click Save the save the method, then close the Method Editor.8.2.3.
Load the corrected method as described above.8.2.4.
To correct the sequence:8.3.
Open the Sequence Editor. In the Sequences box on the left, open 8.3.1.
the folder where the sequence was saved. Click on the sequence to
load it.
Make any corrections as needed (see p. 33).8.3.2.
WARNING
If the probe is not inserted all the way to the bottom of the vessel the Liberty
will not accurately measure the temperature, and signicant overheating of
the vessel will occur. This will result in poor synthesis quality and/or serious
damage to the vessel.