Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
22
Control Buttons
Start: • This button starts the rst method in the queue, or resumes a stopped
method.
Pause: • This button pauses or unpauses the current method.
Stop: • This button stops the current method. PepDriver will nish the operation it
is currently performing before stopping the method.
Menu Buttons
Methods:• This button opens the Method Editor. From the Method Editor, specic
parameters for each synthesis can be programmed.
Sequence:• This button opens the Sequence Editor, where peptide sequences
are entered to be used in methods.
Cycles:• This button opens the Cycle Editor. From the Cycle Editor, cycles can
be created or edited for use in any part of a method.
Setup:
• This menu contains the Microwave Editor, Default Cycle Editor, Pepdriver
Options Menu, and Communication options. From this menu, User Accounts can
also be accessed.
• Maintenance: From this menu, the Maintenance and Diagnostics screens can
be accessed. The Maintenance Screen allows for cleaning, volume calibration,
sensor calibration, and pressure calibration. The Diagnostics Screen contains
information about the sensors, valves, and delay times for the system.
• Calculators: This menu contains the Reagent and Usage calculators. The
Usage Calculator will calculate the amount of each reagent solution needed
for the currently loaded methods. The Reagent Calculator can then be used
to calculate how to make each of the stock solutions for the loaded methods
(including activator, activator base, deprotection, cleavage, and amino acids).
Indicators
Resin Positions:• The twelve positions on the screen correspond to the twelve
positions on the resin manifold of the Liberty. The resin should be loaded in the
same position on the manifold as the method has been loaded into in PepDriver.
Peptide Synthesis Status:• This displays the peptide sequence being
synthesized and indicates the status of the synthesis.
Current Method Detail: • This window displays the currently selected method.
When a method is running, the current step is indicated in yellow.
• System Status Line: The left box indicates the specic command the Liberty
is currently executing. The current temperature and pressure readings are
displayed next to the command indicator. Two indicators shows the computer’s
communication status with the Liberty and the Discover. The waste full and spill
tray warnings are also seen here. On the right, there is an indicator that shows
whether the microwave is running.