Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
48
Add an appropriate amount of DMF to reach the correct nal volume.4.
Place a cap on the bottle. Swirl the bottle gently to ensure all reagents have fully 5.
gone into solution.
Preparing Cleavage Solution
Cleavage is optional. There does not need to be a bottle on the cleavage position unless
cleavage is being performed as part of the loaded methods.
Measure out the appropriate volume of TFA as calculated by the Reagent 1.
Calculator into a 250 mL clear glass bottle.
Add the appropriate volume of the remaining reagents as calculated by the 2.
Reagent Calculator.
Place a cap on the bottle and swirl gently to ensure all the reagents have fully 3.
gone into solution.
Setting up the Liberty
Loading Reagents onto the Liberty
1. Load amino acids onto the Amino Acid Manifolds.
Ensure each dip tube to be used has a dip tube lter in place.1.1.
Remove the caps from each bottle of amino acid. Screw the bottle onto 1.2.
the designated position for that amino acid.
2. Load activator, activator base, and cleavage (if needed) onto the 3-Port Manifold.
Ensure each dip tube has a dip tube lter in place.2.1.
Remove the cap from each bottle. Screw the activator bottle into the top 2.2.
port on the manifold. Screw the activator base bottle into the center port
on the manifold. Screw the cleavage bottle (if needed) into the bottom port
on the manifold.
3. Load deprotection and capping (if needed) using the Change Bottle command.
(For more information about the Change Bottle command, see p. 65.)
Click on the Maintenance button in PepDriver and select Maintenance.3.1.
Select Deprotect from the pull-down box next to the Change Bottle button, 3.2.
then click Change Bottle.
NOTE
Reagent positions that are not used in a given run do not require dip tube
lters or bottles.
WARNING
TFA should only be connected to the Cleavage position. The use of TFA on
any other position will cause serious damage to the Liberty.
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