CEM liberty - Importing a Sequence

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
34
Importing a Sequence
PepDriver includes an option for importing sequences from external documents such as Microsoft
Word documents, PDF documents, or webpages. This is useful for longer sequences because it
minimizes the possibility of incorrectly entering the sequence.
Create a Microsoft Excel spreadsheet containing the sequence or sequences to 1.
be imported.
In Excel, create a new document.1.1.
In cell A1, enter the words SequenceImport (without a space between the 1.2.
words).
Highlight the sequence in the source document (PDF, webpage, etc.) and 1.3.
press Ctrl+C (or right-click and select Copy).
Paste the sequence into the rst open cell in column A of the Excel 1.4.
spreadsheet.
Enter a name for the sequence in column B.1.5.
Repeat steps 1.3 through 1.5 for any other sequences to be imported.1.6.
Save the le and close Excel.1.7.
NOTE
The protected weight refers to the molecular weight of the fully protected
amino acid, and is used in the Reagent Calculator to determine how much of
the amino acid is required for the run.
The unprotected weight refers to the residue weight (the molecular weight
of the fully deprotected amino acid minus 18 g/mol due to the loss of water
from the formation of the peptide bond) and is used in the Sequence Editor
to calculate the total molecular weight for the nal peptide.
For example, Fmoc-Ala-OH has a protected weight of 312 g/mol, and a
residue weight of 71 g/mol:
312 (protected weight) - 223 (Fmoc) - 18 (water) = 71 (unprotected weight)
NOTE
If using Excel 2007 or later, the le must be saved as an Excel 97-2003
compatible le (.xls, not .xlsx). The le name should be in English.