Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
45
The resin position in PepDriver will turn green, indicating the method is loaded 3.
and ready to run.
Loading Multiple Methods
The Liberty is capable of running up to twelve peptide sequentially. When loading multiple
methods, the methods will be run in the order they were loaded. The run priority will be indicated
by the black number in the center of the resin position in PepDriver. The run priority can also be
viewed from the Queue tab.
To change the run priority:
Right-click on a green resin position and select Change Run Priority.1.
Select the new priority.2.
To remove a method from the queue, right-click on the green resin position and select Clear
Method. To empty the queue, right-click on a green resin position and select Clear All Methods.
Preparing Reagents
Standard Concentrations
The Liberty uses stock solutions of all reagents. The default cycles are designed to deliver
enough of each stock solution to give 5 eq of amino acid and activator and 10 eq of activator
base for each coupling. The table below details the standard concentrations used on the Liberty.
0.05 mmol Scale
Concentration
Activator 0.5 M 0.25 M
Activator Base 2 M 1 M
Amino Acid 0.2 M 0.2 M
NOTE
When working on 0.05 mmol scale, due to the size of the sample loops used
for reagent delivery, activator and activator base should be made at half the
standard concentration. Amino acids do not need to be diluted.
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