CEM liberty - Loading Resin Onto the Liberty; Changing the Reaction Vessel; 125 Ml Reaction Vessel

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
50
Loading Resin onto the Liberty
Use the Reagent Calculator to determine the amount of resin required for the 1.
synthesis.
Weigh the appropriate amount of dry resin into a clean 50 mL centrifuge tube.2.
Connect the resin tube onto desired position on the Resin Manifold.3.
If cleavage is being performed, connect a clean, empty 50 mL centrifuge tube 4.
onto the corresponding position of the Product Manifold.
Changing the Reaction Vessel
When working on large or small scales, the reaction vessel will need to be swapped out for one of
an appropriate volume. The large (125 mL) vessel is necessary for reactions of 0.5 mmol scale
or higher. The small (10 mL) vessel is necessary for reactions of 0.05 mmol scale or lower.
125 mL Reaction Vessel
Remove the 35 mL (Standard) reaction vessel from the microwave cavity.1.
Disconnect the ltered drain line from the vessel body.2.
Disconnect the vessel body from the attenuator.3.
Connect the 125 mL vessel body to the attenuator.4.
Connect the ltered drain line to the 125 mL vessel body, being careful not to 5.
crossthread or overtighten the tting.
Place the vessel back into the microwave cavity, securing the attenuator.6.
Perform a Reaction Vessel Leak Check (see p. 94).7.
10 mL Reaction Vessel
Remove the 35 mL (Standard) reaction vessel from the microwave cavity.1.
Carefully remove the temperature probe from the thermowell.2.
Disconnect the four lines from the manifold on the front of the Liberty.3.
Place the 10 mL vessel into the microwave cavity, securing the attenuator.4.
Connect the four line from the 10 mL vessel to the manifold on the front of the 5.
Liberty, ensuring each line is connected to the correct position.
Insert the temperature probe into the thermowell, ensuring the probe is inserted 6.
to the bottom of the thermowell.
Perform a Reaction Vessel Leak Check (see p. 94).7.
NOTE
Some resins will cling to the sides of the tube. If necessary, wash the resin
from the sides of the tube using a small amount (less that 5 mL) of DCM.