CEM liberty - Page 55

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
53
is transferred to the reaction vessel.
The status of the peptide is displayed above the resin positions. In this example,
the resin was preloaded with Fmoc-Gly-OH, so the C-terminal Gly is already
shown on the resin bead with the Fmoc attached. The estimated time remaining
is indicated in the top right. The current step of the method is highlighted in
yellow in the Current Method Display.
The Liberty will enter a wait state for 15 minutes following resin transfer to allow 7.
the resin to swell. The time remaining (in seconds) will be displayed on the
System Status Line.
After the swelling step, the vessel will be drained. The resin will be rinsed 8.
several times, and then the Liberty will begin the deprotection step for the rst
coupling.
Once all couplings are completed, the Liberty will perform the nal deprotection if 9.
one was selected in the Method Editor.
Following nal deprotection (if selected), the Liberty will begin the cleavage cycle.10.
For methods with 10.1. cleavage:
The resin will be rinsed with DCM, and then cleavage cocktail will be 10.1.1.
added from the Cleavage position.
Following the cleavage step, the cleavage solution containing the 10.1.2.
free peptide will be transferred to the corresponding centrifuge tube
on the Product Manifold.
The Liberty will wash the reaction vessel, sending the spent resin out 10.1.3.
to waste.
The Liberty will perform a neutralization procedure on the reaction 10.1.4.
vessel to ensure no residual TFA will interfere with subsequent
syntheses.
The Liberty will perform a reaction vessel cleaning cycle.10.1.5.
For non-cleavage methods:10.2.
Solvent will be added to the reaction vessel followed by bubbling, 10.2.1.
and the resin will be transferred back to the resin tube from which
it was loaded. This will be repeated once to ensure all resin is
returned.
The Liberty will perform a reaction vessel cleaning cycle.10.2.2.
The resin position on the PepDriver main screen will turn blue, indicating the 11.
method was successfully completed.
If multiple methods are loaded, the Liberty will load the next method in the queue 12.
into the Current Run tab, and begin the resin loading cycle for that method.