CEM liberty - Page 66

Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
64
all manifolds on the system. This cleaning only cleans the manifolds and any
internal tubing; it does not affect the dip tubes or any bottles connected to the
system.
• Prime: Clicking this button will prime the lines for the reagent selected from
the pull-down menu.
• Clean RV: Clicking this button begins the reaction vessel cleaning
procedure, and sends all contents of the reaction vessel to waste.
• Neutralization: Clicking this button begins the vessel neutralization
procedure, washing the reaction vessel with deprotection solution followed by
a reaction vessel cleaning step. This command is intended to neutralize any
residual TFA in the vessel following cleavage.
• Resin Return: Clicking this button begins a resin return step, adding main
wash (DMF) and Secondary Wash (DCM) to the reaction vessel and then
transferring the contents to the resin position selected from the pull-down
menu.
• Clean Resin Tube: Clicking this button washes the resin dip tube of the
resin position selected from the pull-down menu to remove any residual
resin.
• Transfer from Resin Tube to RV: Clicking this button begins a resin
transfer step, adding Main Wash (DMF) and Secondary Wash (DCM) to
the resin tube selected from the pull-down menu and then transferring the
contents to the reaction vessel.
• Clean Product Tube: Clicking this button washes the product return tube of
the product position selected from the pull-down menu.
• Wash: Clicking this button adds the selected volume of the selected solvent
(Main Wash/DMF or Secondary Wash/DCM) to the reaction vessel from either
the top or the bottom, then performs a ltered drain.
Drain•
•
Filtered: Clicking this button drains the contents of the reaction vessel
through the glass frit on the bottom of the reaction vessel and through the
inline lter out to waste. Any solid reagents will remain in the reaction vessel.
• Unltered: Clicking this button drains the contents of the reaction vessel
through the resin dip out to waste. This will completely empty the reaction
vessel of all contents (solid and liquid).
• Backush
Backush:• Clicking this button will perform a backush on the reagent(s)
selected from the list. For more information about using the Backush
command, see Performing a Backush, p. 65.
• Backpurge: Clicking this button will perform a backpurge on the reagent(s)
selected from the list.