Liberty
™
Automated Microwave Peptide Synthesizer
600178 • Revision 5 • October 2009
71
Insert the reaction vessel into the calibration stand so that the drain line 1.3.
from the bottom of the vessel ts into the slot on the left of the stand.
From the Volume Calibration tab, click on the Delivery pull-down to select the 2.
reagent to calibrate.
Once a reagent has been selected, click Calibrate to begin the calibration 3.
procedure.
Enter the volume to be used for calibration, then click Add. Typically, 10 mL of 4.
reagent are used for calibration.
CAUTION
When calibrating reagents, do not add Deprotect immediately before or after
DCM. Mixing DCM and Deprotect will cause a solid precipitate to form in
the reaction vessel that can damage the Liberty.
CAUTION
Cleavage should only be calibrated if Boc chemistry is being performed
on the Liberty. After performing a cleavage volume calibration, perform a
reaction vessel neutralization by clicking Neutralization on the Cleaning tab
of the Maintenance screen.
To Next Page
Loading...
