NON-GYNECOLOGIC SAMPLE PREPARATION
1.30
ThinPrep 2000 Processor Operator’s Manual
Mucoid Specimens
Problem Procedure
A. Did the “Sample Is
Dilute” message
appear during pro-
cessing?
1. Check to see if cellularity is adequate. If
not, use more of the pellet if available.
Prepare a slide using Sequence 3.
B. Does the slide have an
obvious “halo” of cel-
lular material and/or
white blood cells?
1. Dilute the sample 20:1. Use a calibrated
pipette to add 1 mL of sample to a new
PreservCyt Solution vial. Prepare slide
using Sequence 1.
If a halo is present on the new slide, call
Hologic Technical Service.
C. Is the slide sparse and
does it contain mucus?
1. Pour the contents of the PreservCyt sam-
ple vial into a centrifuge tube.
Call Hologic Technical
Service.
2. Concentrate by centrifugation.
— 600g x 10 min.
3. Pour off supernatant and vortex to
resuspend cell pellet.
4. CytoLyt Solution Wash
5. Evaluate cell pellet appearance.
Refer to page 1.11.
If pellet contains mucus, repeat from
step 4.
YES
NO
YES
NO
YES
NO
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